biochemical extraction meaning in Chinese

生化提取(法)

Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis . the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme . in studies , it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody . but it is difficult to obtain a single component by biochemical extraction . genetic engineering can help us to desolve this problem . after looking up mrna sequence encoding histidyl - trna synthetase in genebank , we used rt - pcr technology to gain its full length dna sequence . the vestor ptybllwas used in the construction of expressing vestor . we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本实验从人胎盘中提取总rna ，利用rt - pcr技术获得了编码jo - 1的基因整长序列，选用impact - cn系统中的ptyb11载体，构建了jo - 1基因的克隆与表达载体，并转化大肠杆菌er2566 ，经过抗性筛选、分子量大小比较、双酶切鉴定、和pcr鉴定等多种方法验证，筛选出了5个阳性克隆。